Masters of Science

URI for this collectionhttps://rps.wku.edu.et/handle/987654321/9

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    ASSESSMENT ON MILKING AND HANDLING PRACTICES, MICROBIAL QUALITY AND SAFETY OF RAW COW MILK IN KOLFEKERANIO AND LEMIKURA SUB-CITIES OF ADDIS ABABA, ETHIOPIA
    (Wolkite University, 2023-01) KURIBACHEW ENDALE GODANA
    Raw cow milk serves as a good medium for microbial growth due to its complex biochemical composition and high water activity, Because of this character, the quality and shelf-life of milk is forced to be degraded. This study was conducted in Kolfekeranio and Lemikura sub cities of Addis Ababa to evaluate milking practice, microbial quality and safety as well as antimicrobial susceptibility of bacteria in raw cow milk. Two districts were purposively selected from each sub-cities based on dairy potential. Small, medium and large-scale dairy farmers in the selected districts of study area owning dairy cattle for milk production were the targeted population. A total of 161 dairy producers were selected randomly based on proportion from each farm scale and interviewed individually using semi-structured questionnaire. For milk quality evaluation, 61 samples of raw cow milk were collected from producer’s container and transported by icebox to the laboratory. The collected data were analyzed using SPSS software version 25.0. The result showed that the milking method practiced in the study area is hand milking. All the respondents wash their hands before milking but among them 27.3% of them washes with water only and 72.7% of them wash their hand with soap and water. The majority of the respondents use plastic materials for milking (83.9%), and milk storage (68.3%) respectively. The overall mean (±SD) of raw cow’s milk for standard plate count (SPC), E. coli count (EC), Coliform Count (CC) and Total Yeast and Mold Count (TYMC) were 6.24±1.43, 2.44±0.56, 4.34 ±1.27 and 2.87±1.02 log10cfu/ml respectively. Salmonella was not detected in the collected milk sample and the prevalence of Staphylococcus aureus was 37%. The isolated S. aureus bacteria were 100% susceptible for antibiotic drugs like Amikacin, Gentamicin, Kanamycin, Erythromycin, Tylosin, Clindamycin and Florenicol. On the other hand S. aureus was 87% susceptible and 13% intermediate for Enfrofloxacin, 78.3% susceptible and 21.7% resistance for Tilcimosin, 60.9% susceptible and 39.1% resistance for Tetracycline, 95.7% susceptible and 4.3% intermediate for Trimethoprim-Sulfamethoxazole. Based on the result of the current study, milk samples contained higher microbial load than National and International standards and contains pathogenic bacteria which will result in public health risk to the consumer. All concerned bodies in dairy industry should get public education about hygienic milk production to reduce the risk of milk borne pathogens and losses due to rejection resulting from contamination.