Masters of Art

URI for this collectionhttps://rps.wku.edu.et/handle/987654321/8

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    OPTIMIZATION OF COST-EFFECTIVE ENSET (Ensete Ventricosum) (Lemmat) MICROPROPAGATION PROTOCOL UNDER DIFFERENT GELLING AGENT USING MS MEDIA, IN ETHIOPIA
    (WOLKITE UNIVERSITY, 2024-06) MAHILET ABISHU
    Enset (Ensete ventricosum) is culturally, economically and nutritionally vital in Ethiopia, notably in the Gurage region. Traditional propagation methods face challenges like low success rates and disease susceptibility. This study aimed to develop a cost-effective micro propagation protocol for Enset (Lemmat). Shoot tip explants were sterilized with NaOCl and H2O2 solutions at different concentrations: 0%, 1% and 2% for NaOCl and 0%, 10.5%, and 11.5% for H2O2. After sterilization, explants were placed in MS media with 2 mg/l BAP. Initiated shoots were transferred to MS media with varying BAP concentrations (0, 2.5, 3, 3.5 and 4 mg/l) alone and with KN (1 mg/l). Rooting was induced with different IBA concentrations (0, 2, 3 and 4 mg/l) on MS medium. Agar, bulla, sand and glass beads were used to solidify the cultures. Plantlets were acclimatized in various media mixtures in the greenhouse. The sterilization experiment revealed that 40% NaOCl achieved 100% clean and viable explants. Glass beads and agar exhibited superior mean shoot lengths 9.8333 ± 0.29 and 9.6667 ± 0.57 respectively compared to bulla and sand with glass beads 10.0000 ± 0.57 showing the shortest mean duration for shoot initiation. Sands exhibited the highest mean shoot number per explant, as well as the highest mean shoot length 8.67 ± 0.57 on 4mg/l BAP + 1mg/l KN and 9.67 ± 0.57 on 4mg/l BAP + 1mg/l KN respectively for shoot multiplication. Glass beads exhibited the highest mean root length (8.6333 ± 0.50 on 4.0 IBA), while sand showed the highest mean number of roots followed by Sand (6.00 ± 0.57). Acclimatization in a red soil sand and compost mixtures at a 1:1:1 ratio resulted in the highest mean shoot height (26.611 ± 5.86). Utilizing glass beads and sand-based liquid media offers an 80.13% reduction in medium cost per jar, while bulla as a gelling agent saves 84.88% compared to agar. Generally, this study optimizes the micro-propagation of Enset, identifying effective sterilization methods, solidifying agents, and cost-reducing alternatives that enhance growth without compromising quality. Findings suggest broader applications for other plant species to improve agricultural sustainability