PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL EFFECT OF CASTOR BEAN (Ricinus commis) EXTRACT AGAINST ENSET BACTERIAL WILT (Xanthomonas campestris pathovar musacearum

Abstract

Xanthomonas Bacterial wilt attacks enset at any stage, including full maturity. It is reported that up to 80% of enset farms are currently infected by Enset Xanthomonas Wilt. The available genetic diversity of the disease, natures, biology, epidemiology, virulence spectrum of the disease, the host-pathogen interaction, mode of transmission etc. has not been exhaustively studied. Therefore the objective of this study was to extract antibacterial compound from castor bean. Xanthomonas Campestries musacerum was taken from collected sample. The maximum amount of extract was 23gram. Phytochemical extraction and screening from castor bean plant and Xcm controlling efficiency of plant extract was done by dissolving the plant extract with hexane, methanol and chloroform at Wolkite University Molecular laboratory of biotechnology department. As a result inhibition zones are highly formed at extract that dissolve in distilled water while hexane, methanol and chloroform shows less inhibiton than distilled water and some phytochemicals(phenols, terprnoids, tannins, steroid, saponnins, and resin) were identified. Based on the present research finding castor bean extracted by distilled water was shown a maximum inhibition zone which score 1cm at WY1, WY3 and 35 Xcm strain. Due to this result we recommended that castor bean has an antibacterial activity and further advanced researches have to be done to the future to use castor bean as antibacterial agent to control the disease