Senior project on: Isolation, Morphological and biochemical Characterization of local Rhizobium from Root Nodules of Faba Bean, from Guraghe Zone

Abstract

Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Accordingly, the present study will be conducted for the isolation, identification, and characterization of nitrogen-fixing bacterial strains Rhizobium from the legume plant bean which grows widely in almost districts area (Mola kalad, Buchu, Albazer and Koro) of Butajra Guraghe Zone. Nitrogen fixation is the reduction of N2 (atmospheric nitrogen) to NH3 (ammonia) which is made possible by the enzyme nitrogenase. A study was undertaken to investigate the occurrence of rhizobium from the root of beans. Strain of root nodulating bacteria was isolated from a root nodule of faba bean. The isolate were grown on yeast extract Mannitol agar medium and the morphological characterization was realized by gram stain. A total of four isolate were isolated on YEMA medium. The isolates were found circular, colony shape, rod shaped, whitish, entire colony margins and moist colony texture. Gram staining and caseinase test showed negative while carbohydrate utilization tests and catalase test registered positive. All four isolates were showed the characteristics of rhizobia. We recommend that the isolates re- identify, strain type confirmed through molecular characterization techniques and their efficiency should check.