SYNERGISTIC ANTIBACTERIAL ACTIVITY OF GARLIC(Allium sativum) AND NEEM(Azadrichta indica) LEAF EXTRACTS AGAINST SOME SELECTED PATHOGENIC BACTERIA AT WOLKITE UNIVERSITY

Abstract

Medicinal plants have been identified and used throughout human history. Chemical compounds in plants mediate their effect on the human body through process identical to those already will understood for the chemical compounds in conventional drugs thus herbal medicines don’t differ guilty from conventional drugs in terms of how they work. This study was aimed to investigate the antibacterial properties of the mixture of Allium sativa(garlic) and Azadirachta indica (neem) against staphylococcusaureus ,bacilus cereus and Shiga Toxin E.coli . Medicinal plants were extracted with99.9% (v/v) ethanol against tested pathogenic bacteria. MH agar disk and well diffusion method used for antibacterial susceptible tested. Antibiotic disk were used aspositive control .Among the tested pathogenic bacterial both staphylococcus aureusand shiga toxin E.coli were susceptible to crude extracts. Bacillus cereus was resistance to crude extracts of both plant and individual extracts .The inhibition zone of ethanol crude extract of neem and garlic(18 mm) against Shiga toxin E.coli was significantly far greater than S.Aurasis . Natural species of neem and garlic possess effective anti-microbial activity could be used for prevention of pathogenic bacteria and further evaluation was necessary.