College of Agriculture & Natural Resource
URI for this communityhttps://rps.wku.edu.et/handle/123456789/45803
College of Agriculture & Natural Resource
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Item GENETIC DIVERSITY AND POPULATION STRUCTURE ANALYSIS OF SELECTED ETHIOPIAN INDIGENOUS CATTLE BREEDS USING MICROSATELLITE MARKERS(WOLKITE UNIVERSITY, 2023-12) BRUK ALEMU TEKLEThe present study aimed to evaluate genetic diversity, population structure, and level of admixture of four Ethiopian cattle populations namely (Guraghe Gofa , Hamer, and Mursi). Sixteen microsatellite markers were used to assess the genetic diversity of the four ecotypes. Genomic DNA was extracted from bovine blood samples with a genomic DNA extraction salting out method. Ninety-one DNA samples from four (Guraghe (n = 25), Gofa (n = 25), Hamer (n = 25), and Mursi (n = 16)) Ethiopian cattle populations were genotyped. A total of 191 alleles were detected, ranging from 8 to 23 alleles per locus. The mean number of alleles and the observed and expected heterozygosity were 11.9, 0.053, and 0.79, respectively. The FIT, FIS, and FST overall F-statistics have mean values of 0.94, 0.94, and 0.096, respectively. The genetic variation between populations accounted for 9.6% of the total genetic variation. Gene flow (2.56) was observed in allfour populations. Genetic distances, such as PCoA and dendrogram, reveal a close relationship between Hammer and Mursi as compared to Guraghe and Mursi. Factorial correspondence analysis (FCA) and the Structure analysis assigned four Ethiopian populations independently; however, Hamer and Mursi showed a relatively higher degree of admixture than Guraghe and Gofa. It can be concluded that Ethiopia's indigenous cattle populations have a high degree of genetic diversity; These results may be useful in determining current and future breeding programs as well as management and conservation strategies for Ethiopia's indigenous animal genetic resous